Monoclonal antibody against ceramide

This mouse monoclonal IgM antibody is highly specific for ceramide in immuno-overlays of thinlayer-chromatography, in immunohistochemistry and immuno-electron microscopy

Specificity

From left to right: ceramide-1, ceramide-2, ceramide-3, ceramide-5, ceramide C14, ceramide C16, dihydro-ceramide C16, sphingosine, dihydro-sphingosine, glucosylceramid-2, glucosylceramid-3, galactosylceramid-1, galactosylceramid-2, lactosylceramid, cholesterol, sphingomyelin, phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine

Sensitivity

Thinlayer chromatography of various ceramide species and detection by conventional spraying and by immuno-overlay with mab_0011. Chol= cholesterol

Sensitivity in dot-blot

Simultaneous detection of ceramides and cerebrosides in human skin

Double labelling of human skin with monoclonal anti-ceramide antibody (red)
and polyclonal rabbit anti-glucosylceramide (green)

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